![]() ![]() less Figures ( 0) & Videos ( 0)Ĭell heterogeneity is a variation in cellular processes in functionally similar cells. Single-cell western blot has enabled us to detect protein expression at cellular level at a fairly advanced high resolution using a western blot designed to assess cell heterogeneity. For detection of this cell-to-cell variation, a highly advanced technique termed “single-cell western blotting” is carried out. However, it is unable to differentiate protein expression of individual cells. Detection of protein expression from a tissue can be done with the help of western blot. Therefore, off-target and on-target signals can be discriminated. Western blot is done to separate the proteins according to molecular weight. Cross-reactivity can occur leading to bias in result. However, these methods are based on separation by antibodies with limited specificity. Flow cytometry and immunocytochemistry do play an important role in evaluating cell heterogeneity. Single-cell proteome-wide studies are used to detect cell heterogeneity. Cells from the same tissue which are considered genetically identical may have difference in size, structure, and level of protein expression which can lead to major impact on the functions of cell leading to difference in physiological consequences. Cell heterogeneity is a variation in cellular processes in functionally similar cells. ![]()
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